XYLOTROPHIC FUNGUS Trichoderma atroviride: CULTIVATION, EXTRACELLULAR HYDROLYTIC AND ANTIMICROBIAL ACTIVITY

Xylotrophic fungi are well known by their ability to excrete enzymes into environment. These have important biotechnological potential and some of them produce industrial enzymes. Besides, xylotrophic fungal species recently attracted a lot attention among researchers as source antibacterial drugs. Aim. To analyze the effect carbon in culture medium, conditions deep cultivation on mycelium yield, proteolytic, cellulolytic antimicrobial activity liquid Trichoderma atroviride. Methods. Deep methods were used, partial purification was carried out with salting subsequent dialysis, determined spectrophotometrically, using disc diffusion technique. Statistical analysis performed STATISTICA 6.0 software. Results. The highest (0.50±0.03 units/ml), yield smallest colony diameter detected when cellulose used source. However, proteolytic observed glucose optimal temperature range for hydrolase shown be 25-30 °C. In comparison Pleurotus ostreatus, T. atroviride not only has more pronounced activity, but also inhibits growth Candida albicans. Conclusions. isolated strain is promising hydrolytic that can organic farming industry. purified preparation obtained from showed significant successfully drug development future.